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EvaGreen® 20X in Water, 1 mL

EvaGreen® 20X in Water, 1 mL

Quick Overview:

Recommended use in Covid-19, coronavirus, SARS-CoV-2, 2019-nCoV research

Product Code: 31000-T
Supplier: Biotium

  • Details

    EvaGreen dye is a green fluorescent nucleic acid dye with features that make the dye useful for several applications including qPCR and DNA melt curve analysis, real-time monitoring of thermophilic helicase-dependent amplification (tHDA), and capillary gel electrophoresis. The dye is essentially nonfluorescent by itself, but becomes highly fluorescent upon binding to dsDNA. The DNA-bound dye has excitation and emission spectra very close to those of fluorescein (FAM) or SYBR Green I, making it readily compatible with instruments equipped with the 488 nm argon laser or any visible light excitation with wavelength in the region. EvaGreen dye is extremely stable both thermally and hydrolytically, providing convenience during routine handling. EvaGreen dye is nonmutagenic and noncytotoxic because it is impermeable to cell membranes, unlike SYBR Green I, which enters cell rapidly and is known to be a powerful mutation-enhancer. The unique properties of EvaGreen dye have made it particularly useful in quantitative real-time PCR (qPCR) applications. Compared with the widely used SYBR Green I, EvaGreen dye is generally less inhibitory toward PCR and less likely to cause nonspecific amplification. As a result, EvaGreen dye can be used at a much higher dye concentration than SYBR Green I, resulting in a high resolution signal for droplet digital PCR, real-time PCR and melt curve analysis. Recommended use in Covid-19, coronavirus, SARS-CoV-2, 2019-nCoV research. LAMP PCR COVID-19 with EvaGreen. A Single and Two-Stage, Closed-Tube, Molecular Test for the 2019 Novel Coronavirus (COVID-19) at Home, Clinic, and Points of Entry. (Published: https://doi.org/10.26434/chemrxiv.11860137.v1)
  • Additional Information

    Supplier name Biotium
    Product Type DNA & RNA
    Size 1 ML
    Publications Publication List
    Datasheet URL Contact info@jlresearch.com.au for further information.
    Categories DNA & RNA,PCR & Real-Time PCR,COVID-19 Products
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