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Quick Overview:

Product Code: hkb-isgkostg
Supplier: InvivoGen

  • Details

    STING Knockout IRF Reporter Cell Line. HEK-Blue™ ISG-KO-STING cells were specifically designed to study the STING/TBK1/IRF3 signaling pathway. HEK-Blue™ ISG-KO-STING cells were generated from the HEK-Blue™ ISG cells through the stable knockout of the STING gene. The parental HEK-Blue™ ISG cells were derived from the PEAKrapid cell line (similar to ATCC® CRL-2828™) which itself was derived from the HEK293 cell line. Unlike their parental cell line, HEK-Blue™ ISG-KO-STING cells do not respond to cytosolic DNA, CDNs and xanthenone derivatives, such as DMXAA. HEK-Blue™ ISG-KO-STING cells can detect bioactive type I IFNs through the activation of the JAK-STAT-IRF9 pathway. HEK-Blue™ ISG-KO-STING cells express a secreted embryonic alkaline phosphatase (SEAP) under the control of the IRF-inducible promoter comprised of five IFN-stimulated response elements (ISRE) fused to an ISG54 minimal promoter. Levels of SEAP in the supernatant can be easily determined with QUANTI- Blue™, a reagent that turns purple/blue in the presence of SEAP and by reading the OD at 620-655 nm.
  • Additional Information

    Supplier name InvivoGen
    Product Type Cell & Tissue Culture
    Size 3-7 x 10e6 cells
    Publications Publication List
    Datasheet URL View Datasheet
    Categories Cell & Tissue Culture,Cell Lines,COVID-19 Products
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