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Quick Overview:

Product Code: hkb-isg-1
Supplier: InvivoGen

  • Details

    Interferon regulatory factor (IRF)-inducible SEAP reporter HEK293 cells. HEK-Blue™ ISG cells were specifically designed to study the activation of the STING/TBK1/IRF3 signaling pathway by CDNs. HEK-Blue™ ISG cells were derived from the PEAKrapid cell line (similar to ATCC® CRL-2828™) which itself was derived from the HEK293 cell line. HEK-Blue™ ISG cells express a secreted embryonic alkaline phosphatase (SEAP) under the control of the IRF-inducible promoter comprised of five IFN-stimulated response elements (ISRE) fused to an ISG54 minimal promoter. CDNs in the cytosol bind directly to STING leading to TBK1-mediated IRF3 activation and type I IFN production. IFNs activate the JAK-STAT pathway with the subsequent activation of IFN-stimulated response elements (ISRE) in the promoters of IFN-stimulated genes (ISG). Hence, the presence of CDNs in the cytosol of HEK-Blue™ ISG cells will induce the production of the IRF-inducible SEAP reporter directly by activating the STING/TBK1/IRF3 pathway and indirectly through the activation of the JAK/STAT/IRF9 pathway with type I IFN. Levels of SEAP in the supernatant can be easily determined with QUANTI- Blue™, a reagent that turns purple/blue in the presence of SEAP and by reading the OD at 620-655 nm. HEK-Blue™ ISG cells respond strongly to non-canonical CDNs, namely 2’3’-cGAMP and 2’2’-cGAMP but poorly to cytosolic DNA, DMXAA and canonical cyclic dinucleotides (CDNs).
  • Additional Information

    Supplier name InvivoGen
    Product Type Cell & Tissue Culture
    Size 3-7 x 10e6 cells
    Publications Publication List
    Datasheet URL View Datasheet
    Categories Cell & Tissue Culture,Cell Lines,COVID-19 Products
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