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Product Code: thp-gb1lc
Supplier: InvivoGen

  • Details

    Pyroptosis and necroptosis reporter monocytes. THP1-HMGB1-Lucia™ cells are designed to monitor necroptosis and inflammasome-mediated pyroptosis, two forms of necrotic cell death characterized by the release of the alarmin HMGB1 (High Mobility Group Box-1) upon cell membrane rupture. Pyroptosis is a consequence of inflammasome activation. Typically, inflammasome activation is a two-step process. A first signal (‘priming’), provided by microbial molecules such as lipopolysaccharide (LPS), induces NF-κB-dependent expression of pro-IL1β. The second signal, provided by structurally unrelated microbial molecules (e.g. Nigericin toxin) or danger signals, triggers inflammasome multimerization. This leads to caspase-1 self-activation, proteolytic maturation of IL-1β and IL-18, and cleavage of Gasdermin D (GSDMD). Subsequent GSDMD pore formation at the cell membrane elicits a rapid cell death associated with the release of IL-1β, IL-18 and HMGB1 [1,2]. Necroptosis is mainly implicated in conditions of disease or infection and is triggered by the formation of a necrosome. The necrosome is induced upon Tumor Necrosis Factor (TNF) receptor or PRR activation in absence of caspase-8 and cIAP (cellular inhibitor of apoptosis) activity. The necrosome contains receptor-interacting serine/threonine-protein kinases RIPK1 and RIPK3, and the mixed lineage kinase-like MLKL. Phosphorylated MLKL relocalizes at the plasma membrane where it drives cell lysis and release of HMGB1, most probably through pore formation [3]. THP1-HMGB1-Lucia™ cells are derived from the THP-1 human monocytic cell line and stably express a HMGB1::Lucia-luciferase fusion protein. These cells respond to commonly used inflammasome inducers and necroptosis cocktail inducers. The release of the HMGB1::Lucia protein in the extracellular milieu upon pyroptosis or necroptosis can be readily measured using the QUANTI-Luc™ detection reagent. Levels of IL-1β can be measured by Western blot, ELISA or using InvivoGen’s HEK-Blue™ IL-1β cellular assay. Alternatively, secreted IL-18 can be detected using InvivoGen’s HEK-Blue™ IL-18 cellular assay. THP1-HMGB1-Lucia™ cells are resistant to Zeocin™. References: 1. Broz P. and Dixit V.M., 2016. Inflammasomes: mechanism of assembly, regulation and signalling. Nat Rev Immunol. 16:407-20. 2. Kovacs S.B. and Miao E.A., 2017. Gasdermins: effectors of pyroptosis. Trends Cell Biol. 27:673-84. 3. Grootjans S. et al., 2017. Initiation and execution mechanisms of necroptosis: an overview. Cell Death Differ. 24:1184-95.
  • Additional Information

    Supplier name InvivoGen
    Product Type Cell & Tissue Culture
    Size 3-7 x 10e6 cells
    Publications Publication List
    Datasheet URL View Datasheet
    Categories Cell & Tissue Culture,Cell Lines,COVID-19 Products
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